Madonna in Glory with Seraphim is a painting by the Italian Renaissance painter Sandro Botticelli, executed c. 1469-1470. It is housed in Galleria degli Uffizi, Florence.

Some panel paintings of the Madonna by Botticelli remain from the years around 1470 which are striking expressions of Botticelli's stylistic development. Two full figure pictures of the Madonna - the Madonna in Glory and the Madonna of the Rosegarden - are in the Uffizi. They represent monumental seated figures filling the entire picture.

Mary is enthroned on clouds in a glory of seraphim. The Christ Child, with the cruciform nimbus, is looking towards the observer and raising his hand in blessing. Botticelli has succeeded in expressing the tensions in this theme with sensitivity: the mother, who is fully aware of the Passion her son will suffer, is holding him protectively in her arms.

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